Rat Anti-SARS-CoV-2 Nucleoprotein IgG ELISA Kit

Sample

serum

Species Reactivity

Rat

Intended Use

The Rat Anti-SARS-CoV-2 (Covid-19) IgG Antibody to Nucleoprotein Quantitative TITRATION ELISA kit is used as an analytical tool for quantitative estimation of Anti-SARS-CoV-2 (2019-nCoV) Nucleoprotein antibodies in rat serum.

Storage

1. Store main kit components at 2-8°C.
2. Before using, bring all components to room temperature (18-25°C). Upon assay completion return all components to appropriate storage conditions.
3. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Precision

Precision is defined as the percent coefficient of variation (%CV) i.e. standard deviation divided by the mean and multiplied by 100. Assay precision was determined by both intra (n=5 assays) and inter assay (n=5 assays) reproducibility on two pools. While actual precision may vary from laboratory to laboratory and technician to technician, it is recommended that all operators achieve precision below these design goals before reporting results.

Detection Limit

There is no standard reference rat SARS-CoV-2 nucleoprotein material available; accordingly, absolute analytical sensitivity cannot be calculated. Based on the kit working standards the LOD is 12 ng/ml.

Sensitivity

Mutations in the SARS-CoV-2 genome have been identified as the virus has spread, but no serologically unique strains have been described relative to the originally isolated virus (this research is limited at present). The kit detects antibodies specific to the Nucleocapsid protein of SARS-CoV-2 virus.