This kit can be used for quantitative measurement of anti-PEG IgM in rabbit serum and plasma.
Contents of Kit
No.
Components
Size
Storage Conditions
1
PEG-BSA Coated Plate
12×8 wells
-20°C
2
Anti-Rabbit IgM HRP Stock (lyophilized)
1
-20°C
3
Reference Stock (lyophilized)
1
-20°C
4
20× HRP PEG Wash
50 ml
2-8°C
5
HRP PEG Diluent
50 ml
2-8°C
6
TMB
11 ml
2-8°C
7
Stop Solution
11 ml
2-8°C
Storage
The reference stock, HRP conjugate, and the PEG-BSA coated plate should be stored at - 20°C. All remaining kit components should be 1Anti-PEG IgM levels are measured in nominal units and are calibrated using pooled anti-PEG serum. stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant. Kits will remain stable for six months from the date of purchase provided that the components are stored as described.
Performance Characteristics
Parallelism: To assess performance of the assay, three samples containing anti-PEG IgM at concentrations of 44,886, 193,233 and 368,990 u/ml were serially diluted to produce values within the dynamic range of the assay.
Standard Curve
A typical standard curve with optical density readings at 450nm on the Y-axis against anti-PEG IgM concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns.
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