Rabbit Anti-Haemophilus Influenzae B (HIB) IgG ELISA Kit

Sample

Serum

Species Reactivity

Rabbit

Intended Use

This kit is intended to detect anti-Haemophilus Influenzae B IgG antibody in rabbit serum post vaccination.

Storage

All reagents should be stored at 2-8°C , and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8°C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Opened pouches should be securely resealed by folding over the open end and securing it with adhesive tape.
The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8°C. It is recommended that Wash buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.
Indications of Deterioration
The Rabbit Anti-Haemophilus influenzae type b Assay may be considered to have deteriorated if:
1. The kit fails to meet the required criteria for a valid test (see Interpretation of Results).
2. Reagents becoming visibly cloudy or develop precipitate. Note: Concentrated Wash buffer, when cold, normally develops crystalline precipitates, which re-dissolve on heating at 37°C.
3. The Substrate Solution turns dark green. This is likely to be caused by contamination of the Substrate Solution.

General Description

Haemophilus influenzae type b can cause bacteremia, pneumonia, and acute bacterial meningitis in infants and young children. Vaccines have been developed with Haemophilus influenzae type b polysaccharide conjugated to a protein to protect from infection. Antibodies should be detectable in animals post vaccination.