Purified Human IgG1 Isotype Control Recombinant Antibody (Low Endotoxin, Azide-Free) (DAG-IC229) Functional Grade

Purified Human IgG1 Isotype Control Recombinant Antibody (Low Endotoxin, Azide-Free) for FC, ELISA

Additional Formats Available

Specifications


Host Species
Human
Antibody Isotype
IgG1, κ
Clone
RB27B23
Species Reactivity
N/A
Conjugate
Functional Grade

Applications


Application Notes
FC, ELISA
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

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References


Ginsenoside Rg1 inhibits the TSLP production in allergic rhinitis mice

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY

Authors: Oh, Hyun-A; Seo, Ji-Young; Jeong, Hyun-Ja; Kim, Hyung-Min

Recent study reports that Korean red ginseng reduces the nasal allergic inflammatory reaction in an allergic murine model. However, the contribution of ginsenoside Rg1 (RG1) and its mechanisms on allergic rhinitis (AR) have not been elucidated. In this study, we evaluated the important activities of RG1 in the ovalbumin (OVA)-induced AR mice. RG1 significantly reduced the levels of thymic stromal lymphopoietin (TSLP) and interleukin (IL)-1 beta compared with the AR control mice. Allergic symptom such as rub scores and biomarkers such as spleen weight, histamine, IgE and IgG1 in the RG1 group were decreased compared with the AR mice. The levels of interferon-g were enhanced while the levels of IL-4 were reduced in the RG1 group. In the RG1 group, the eosinophils and mast cells infiltration increased by OVA were also decreased. RG1 reduced the levels of inflammation-related protein. RG1 inhibited the caspase-1 activity in nasal mucosa tissue. In addition, RG1 inhibited the production of TSLP and IL-1 beta and the activations of caspase-1, receptor interacting protein 2, I kappa B kinase-beta and nuclear factor-kappa B/Rel A in activated HMC-1 cells. Our results indicate that RG1 has the inhibitory effect of TSLP production and caspase-1 activity in AR experimental model.

Maintenance of tolerance to cow's milk in atopic individuals is characterized by high levels of specific immunoglobulin G4

CLINICAL AND EXPERIMENTAL ALLERGY

Authors: Ruiter, B.; Knol, E. F.; van Neerven, R. J. J.; Garssen, J.; Bruijnzeel-Koomen, C. A. F. M.; Knulst, A. C.; van Hoffen, E.

Background The central role of specific IgE in cow's milk allergy (CMA) is well documented. However, less is known about the function of other immunoglobulin isotypes in allergy and tolerance to cow's milk proteins (CMPs). Objective To determine differences in the antibody responses that are associated with allergy and tolerance to cow's milk in allergic, atopic and non-atopic individuals of different age groups. Methods Nineteen infants (< 1 year), 18 children (6-14 years) and 41 adults (21-68 years) were included. Each age group was comprised of subjects with CMA, atopic individuals without a history of CMA and non-atopic subjects. Levels of specific IgE, IgG4, IgG1 and IgA to whole cow's milk and the six most abundant individual CMPs were determined in plasma by ELISA. For comparison, specific IgE and IgG4 were measured to ovomucoid and house dust mite (HDM) in individuals allergic for the respective allergens, and in atopic and non-atopic subjects without allergy. Results In infants and children with CMA, alpha s1-casein and beta-lactoglobulin induced the highest specific IgE response, whereas alpha s1-casein was the most allergenic CMP in adult patients. Specific IgG4 and IgG1 responses were the highest to alpha s1-casein and beta-lactoglobulin in all age groups, while kappa-casein and alpha-lactalbumin induced the highest levels of IgA. CMP-specific IgG4 was higher in atopic children and adults without CMA, as compared with non-atopic individuals. A similar difference between tolerant atopic and non-atopic subjects was observed for IgG4 specific to ovomucoid, whereas HDM-specific IgG4 was not detectable in these subjects. Conclusions Maintenance of tolerance to cow's milk in atopic children and adults without CMA is associated with elevated levels of specific IgG4, in combination with low specific IgE. The up-regulation of specific IgG4 in tolerant atopic individuals may be related to the type of allergen and its regular dose of exposure.

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