Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase (UK variant B.1.1.7, 20I/501Y.V1, Alpha)

Product Overview

Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase (UK variant B.1.1.7, 20I/501Y.V1, Alpha) encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with the firefly luciferase (Fluc) reporter gene and SARS-CoV-2 spike protein (ΔH69/V70, ΔY144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) is incorporated as the membrane protein on the surface of the VSV pseudotyped virus. Because the infectivity of Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. The pseudotype VSV particles encode luciferase together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, luciferase expression is proportional to the number of cells that were infected

Nature

Virus

Storage

Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80 ° C refrigerator and melt it in an ice bath.

Ship

Frozen on dry ice