Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase (Indian variant B.1.617.1, Kappa) encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with the firefly luciferase (Fluc) reporter gene and SARS-CoV-2 spike protein (G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H) is incorporated as the membrane protein on the surface of the VSV pseudotyped virus. Because the infectivity of Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. The pseudotype VSV particles encode luciferase together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, luciferase expression is proportional to the number of cells that were infected
Biosafety Level: BSL-2
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