This pseudovirus features the VSV-ΔG core pseudotyped with NiV G and F envelope proteins and encoding green fluorescent protein (GFP). The bright green fluorescence (excitation/emission: 488/507 nm) provides excellent signal intensity for cellular and tissue-level imaging studies. As the most commonly used fluorescent reporter, GFP offers broad compatibility with existing microscopy infrastructure and standard FITC filter sets, making this product accessible for routine laboratory screening and educational purposes. Note: 1. Biosafety: These are BSL-2 reagents; they are replication-defective and capable of only single-round infection 2. Storage: -80°C for long-term; avoid repeated freeze-thaw cycles (>3 cycles not recommended) 3. Cell lines: Vero, Vero E6, and PK13 cells are standard targets for NiV-G/F entry studies; verify receptor expression (e attending BSB, Ephrin-B2/B3) 4. Titration: End-users should perform pilot experiments to determine optimal challenge doses for their specific cell models and applications
Store at -80°C. Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80°C refrigerator and melt it in an ice bath.
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