Uricase Assay Kit

Name: Uricase Assay Kit
SKU: DFLA26233
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Tissue extracts, Cell lysate, Cell culture media, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Uricase (UO) activity in tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Substrate	Powder x 1	4°C
Substrate Diluent	1 mL x 1	4°C
Reaction Buffer	10 mL x 1	4°C
Enzyme	Powder x 1	-20°C
Dye Reagent	Powder x 1	-20°C, keep in dark
Standard ( mmoI/L)	3	1 mL x 1	4°C
Positive Control	Powder x 1	-20°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.03 mmol/L - 3 mmol/L

General Description

Uricase (EC 1.7.3.3) is an enzyme involved in the uric acid metabolism. Uric acid is the product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings. Uricase Microplate Assay Kit provides a simple and direct procedure for measuring uricase activity in a variety of samples. The assay is initiated with the enzymatic catalysis of the uric acid by uricase. The enzyme catalyzed reaction products quinone can be measured at a colorimetric readout at 550 nm.

Citations