Tylosin ELISA Kit

A simple technique for the isolation, identification, and determination of 12 macrolide antibiotics (azithromycin, roxithromycin, josamycin, spiramycin I-III, tilmicosin, tylosin, erythromycin, clarithromycin, tylvalosine, and tulathromycin) in raw food materials and food products is developed based on high-resolution time-of-flight mass spectrometry. The range of the detected macrolide content is 1-400 ng/g, the limits of detection are 0.01-0.50 ng/g, and the limits of quantification are 0.04-2 ng/g. A scheme for identification and quantification of the detected antibiotics using the standard addition method is proposed. The relative standard deviation of the analysis does not exceed 21%. The duration of the sample screening is 40 min and the time required for quantification of the detected antibiotics is 1.0-1.5 h.

Widespread antibiotic use and the antimicrobial resistance phenomenon demand new analytical methods and the use of non-conventional matrices increasingly necessary for safe food control. We present a method developed to detect six common antibiotics used in poultry breeding, in the unconventional matrix, feathers, compared to muscle and liver. The analysis for the presence of two beta-lactams (penicillin V, amoxicillin), two fluoroquinolones (enrofloxacin, ciprofloxacin), one phenicol (thiamphenicol) and one macrolide (tylosin) was validated and achieved by HPLC-HRMS, with the ultimate aim to identify untargeted metabolites in broilers subjected to different therapeutic protocols. All the validated method parameters met the regulatory requirements. Muscle and liver were not effective matrices when the withdrawal periods were largely respected. Conversely, feathers proved a promising matrix for the detection of all the studied antibiotics, in the range of 8.72-1885.32 ng g(-1), except penicillin V. Like other nonconventional matrices, such as teeth, the antibiotics detected in feathers existed in their unmetabolised form. (C) 2018 Elsevier Ltd. All rights reserved.