Sample
Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Tryptophan content in serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer I | 30 mL x 2 | 4°C |
| Assay Buffer II | 30 mL x 2 | 4°C |
| Dye Reagent | Powder x 1 | 4°C |
| Dye Reagent Diluent | 18 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Plate Sealer | 3 | |
| Technical Manual Manual | 1 | |
Detection Range
0.05 mmol/L - 10 mmol/L
General Description
Tryptophan is an α-amino acid that is used in the biosynthesis of proteins. Tryptophan contains an α-amino group, an α-carboxylic acid group, and a side chain indole, making it a non-polar aromatic amino acid. It is essential in humans, meaning the body cannot synthesize it; it must be obtained from the diet. Tryptophan is also a precursor to the neurotransmitter serotonin, the hormone melatonin and vitamin B3. Tryptophan is important to the functions of many organs in the body. When you consume tryptophan, your body absorbs it and changes it to eventually become a hormone called serotonin. Tryptophan Microplate Assay Kit provides a convenient tool for sensitive detection of Tryptophan in a variety of samples. The intensity of the product color, measured at 600 nm, is proportional to the Tryptophan concentration in the sample.
Citations
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