Trypsin Assay Kit

Name: Trypsin Assay Kit
SKU: DFLA26080
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Trypsin activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Reaction Buffer	10 mL x 1	4°C
Substrate	Powder x 1	4°C
Substrate Diluent	1.2 mL x 1	Room temperature
Stop Solution	10 mL x 1	4°C
Standard ( umol/L)	500	1 mL x 1	4°C
Positive Control	Powder x 1	-20°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

5 umol/L - 500 umol/L

General Description

Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. The assay is initiated with the enzymatic catalysis of the BAEE by Trypsin. The enzyme catalyzed reaction products BA can be measured at a colorimetric readout at 405 nm.

Citations