T2 Toxin ELISA Kit

Name: T2 Toxin ELISA Kit
SKU: DEIA6851
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

feedstuff, feed, grain

Detection Method

cELISA

Intended Use

This immunoassay kit allows for the in vitro quantitative determination of T-2 toxin concentrations in feedstuff, feed, grain.

Contents of Kit

1. Assay plate, 96T
2. Standard, 5×1mL. S1 0 μg/kg, S2 0.3 μg/kg, S3 0.9 μg/kg, S4 2.7 μg/kg, S5 8.1 μg/kg
3. HRP-conjugate, 1×7mL
4. Antibody, 1×7mL
5. TMB Substrate, 1×12mL
6. Stop Solution, 1×10 mL
7. Sample Diluent, 2×50 mL
8. Wash Buffer(10×), 1×50 mL
9. Adhesive Strip, 4
10. Instruction Manual, 1

Storage

Unopened kit: Store at 2--8°C. Do not use the kit beyond the expiration date
Opened kit: May be stored for up to 1 month at 2-8°C.

Precision

Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

0.3 μg/kg-8.1 μg/kg

Detection Limit

Feedstuff, feed, grain 10 μg/kg

Sensitivity

The minimum detectable dose of the kit is typically less than 0.3 μg/kg.
The sensitivity of this assay, or Lower Limit of Detection (LOD) was defined as the lowest concentration that could be differentiated from zero. It was determined the mean OD value of 20 replicates of the zero standard added by their three standard deviations.

General Description

T-2 toxin is mycotoxin produced by several Fusarium species. Food crops as wheat, barely, corn, and their products are mainly contaminated. T-2 toxin is a threat for human health and animal husbandry. T-2 toxin primarily affects the blood, liver, kidneys, pancreas, muscles and function of the lymphocyte. The general symptoms of T-2 toxin poisoning are anorexia, vomiting, diarrhea, growth retardation, reproduction and neurological dysfunction.
This kit is a detection product developed based on ELISA technology, with operation time as short as 25 min and a sensitivity of 0.3 μg/kg, and linear range from 0.3 μg/kg to 8.1 μg/kg.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Worldwide contamination of food-crops with mycotoxins: Validity of the widely cited 'FAO estimate' of 25%

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION

Authors: Eskola, Mari; Kos, Gregor; Elliott, Christopher T.; Hajslova, Jana; Mayar, Sultan; Krska, Rudolf

Abstract

Prior to 1985 the Food and Agriculture Organization (FAO) estimated global food crop contamination with mycotoxins to be 25%. The origin of this statement is largely unknown. To assess the rationale for it, the relevant literature was reviewed and data of around 500,000 analyses from the European Food Safety Authority and large global survey for aflatoxins, fumonisins, deoxynivalenol, T-2 and HT-2 toxins, zearalenone and ochratoxin A in cereals and nuts were examined. Using different thresholds, i.e. limit of detection, the lower and upper regulatory limits of European Union (EU) legislation and Codex Alimentarius standards, the mycotoxin occurrence was estimated. Impact of different aspects on uncertainty of the occurrence estimates presented in literature and related to our results are critically discussed. Current mycotoxin occurrence above the EU and Codex limits appears to confirm the FAO 25% estimate, while this figure greatly underestimates the occurrence above the detectable levels (up to 60-80%). The high occurrence is likely explained by a combination of the improved sensitivity of analytical methods and impact of climate change. It is of immense importance that the detectable levels are not overlooked as through diets, humans are exposed to mycotoxin mixtures which can induce combined adverse health effects.

Mycotoxin profiling of 1000 beer samples with a special focus on craft beer

PLOS ONE

Authors: Peters, Jeroen; van Dam, Ruud; van Doorn, Ronald; Katerere, David; Berthiller, Franz; Haasnoot, Willem; Nielen, Michel W. F.

Abstract

Currently beer is booming, mainly due to the steady rise of craft breweries worldwide. Previous surveys for occurrence of mycotoxins in beer, were mainly focussed on industrial produced beer. The present survey reports the presence of mycotoxins in craft beer and how this compares to industrial produced beer. More than 1000 beers were collected from 47 countries, of which 60% were craft beers. A selection of 1000 samples were screened for the presence of aflatoxin B-1, ochratoxin A (OTA), zearalenone (ZEN), fumonisins (FBs), T-2 and HT-2 toxins (T-2 and HT-2) and deoxynivalenol (DON) using a mycotoxin 6-plex immunoassay. For confirmatory analysis, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and applied. The 6-plex screening showed discrepancies with the LC-MS/MS analysis, possibly due to matrix interference and/or the presence of unknown mycotoxin metabolites. The major mycotoxins detected were DON and its plant metabolite deoxynivalenol-3-beta-D-glucopyranoside (D3G). The 6-plex immunoassay reported the sum of DON and D3G (DON+D3G) contaminations ranging from 10 to 475 mu g/L in 406 beers, of which 73% were craft beers. The popular craft beer style imperial stout, had the highest percentage of samples suspected positive (83%) with 29% of all imperial stout beers having DON+D3G contaminations above 100 mu g/L. LC-MS/MS analysis showed that industrial pale lagers from Italy and Spain, predominantly contained FBs (3-69 mu g/L). Besides FBs, African traditional beers also contained aflatoxins (0.1-1.2 mu g/L). The presence of OTA, T-2, HT-2, ZEN, beta-zearalenol, 3/15-acetyl-DON, nivalenol and the conjugated mycotoxin zearalenone 14-sulfate were confirmed in some beers. This study shows that in 27 craft beers, DON+D3G concentrations occurred above (or at) the Tolerable Daily Intake (TDI). Exceeding the TDI, may have a health impact. A better control of brewing malts for craft beer, should be put in place to circumvent this potential problem.