Lactose Assay Kit

Name: Lactose Assay Kit
SKU: DFLA26231
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Urine, Saliva, Milk, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Glucose content in serum, plasma, urine, saliva, milk, tissue extracts, cell lysate, cell culture and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer I	30 mL x 1	4°C
Assay Buffer II	30 mL x 1	4°C
Enzyme I	Powder x 1	-20°C
Enzyme II	Powder x 2	-20°C
Reaction Buffer	15 mL x 1	4°C
Dye Reagent	Powder x 2	4°C, keep in dark
Standard	Powder x 1	4°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.2 mmol/L - 20 mmol/L

General Description

Lactose (C H O ), also called milk sugar, is a disaccharide that consists 12 22 11 of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2-8% of milk. Lactose Microplate Assay Kit provides a simple and direct procedure for measuring lactose levels in a variety of samples. Lactose is hydrolysed by lactase (β- galactosidase), released galactose and glucose. Then glucose can be hydrolysed by glucose oxidase. The enzyme catalyzed reaction product H O can be measured at a colorimetric readout at 505 nm. 2 2

Citations