Histamine Dehydrogenase Activity Assay Kit

Name: Histamine Dehydrogenase Activity Assay Kit
SKU: DFLA26270
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Tissue extracts, Cell lysate, Cell culture media and Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Histamine Dehydrogenase (HDH) activity in tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Assay Buffer	30 mL x 4	4°C
Reaction Buffer	15 mL x 1	4°C
Substrate	Powder x 1	4°C
Dye Reagent A	Powder x 1	4°C
Dye Reagent B	1 mL x 1	4°C
Standard	Powder x 1	4°C
Positive Control	Powder x 1	-20°C
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.01 mmol/L - 1 mmol/L

General Description

Histamine dehydrogenase is a homodimeric enzyme that catalyzes oxidative deamination of histamine. Histamine dehydrogenase can be isolated from cultures of Nocardiodes simplex grown on histamine as the sole nitrogen source. The enzyme is a homodimer of a ∼76kDa subunit responsible for catalyzing the oxidative deamination of histamine to give imidazole acetaldehyde (Scheme 1), where the enzyme exhibits remarkable selectivity for histamine, thus showing great potential for use in biosensors. HADH was originally classified as a quinone-containing amine dehydrogenase. Later studies revealed that HADH is a homologue of TMADH, and dimethylamine dehydrogenase (DMADH) from M. methylotrophus shares 40% sequence identity and 56% similarity with both proteins. Histamine Dehydrogenase Activity Microplate Assay Kit is a sensitive assay for determining histamine dehydrogenase activity in various samples. Histamine is hydrolyzed by histamine dehydrogenase. The intensity of product color, measured at 450 nm is directly proportional to histamine dehydrogenase activity in the sample.

Citations