Application Notes
The binding reactivity of this antibody towards Dxd-KLH and Dxd-BSA conjugates was determined using ELISA. This antibody is capable of binding antibody-drug conjugates ADC-8201 in a competitive ELISA, wherein the antibody was trastuzumab. The binding affinity of this antibody towards Dxd-BSA was determined using surface plasmon resonance (SPR) and it was reported to be Kd= 3.38nM
General Notes
The Hi-Puri™ Mouse Anti-DXD Monoclonal Antibody, HRP-conjugated (clone 12G9-2, product code CABT-L1909W) is a mouse monoclonal antibody specifically directed against DXD (Dxd), also known as an exatecan derivative or camptothecin analog. DXD is a potent cytotoxic small-molecule payload derived from camptothecin that functions as a topoisomerase I (TOP1) inhibitor and is widely used in antibody-drug conjugate (ADC) development. By forming a stable ternary complex with TOP1 and DNA, DXD blocks DNA replication, induces cell cycle arrest, and promotes cancer cell death. DXD-containing ADCs, including HER2-targeting DXD conjugates, are important platforms for targeted delivery of cytotoxic agents in cancer research. This antibody was generated by immunizing BALB/c mice with DXD-KLH conjugate and is suitable for research applications involving DXD detection and ADC characterization.
This product is validated for use in ELISA applications and is conjugated with HRP for direct signal detection. The binding reactivity of this antibody toward DXD-KLH and DXD-BSA conjugates was determined using ELISA. The antibody is capable of recognizing DXD-containing antibody-drug conjugates in competitive ELISA formats, including ADC-8201-related applications. The binding affinity toward DXD-BSA was determined using surface plasmon resonance (SPR), with a reported affinity of Kd = 3.38 nM. This antibody specifically reacts with DC-8201 and shows no reaction with ADC029, supporting selective detection of DXD-containing ADC molecules.
CABT-L1909W is a mouse IgG monoclonal antibody purified by affinity purification and conjugated with HRP. It is supplied as a liquid formulation in PBS and available in multiple sizes, including 100 μg, 200 μg, 500 μg, and 1 mg. For long-term storage, the product should be stored at −20°C and repeated freeze–thaw cycles should be avoided. The product is shipped on dry ice. This product is intended for research use only and is not intended for diagnostic use.
COA
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