Human ERBB2 ELISA Kit

Sample

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For quantitative detection of human ERBB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Contents of Kit

1. Anti-Human ERBB2 Pre-coated 96-well Strip Microplate: 1, 12 strips of 8 wells Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 4°C provided this is within the expiration date of the kit.
2. Human ERBB2 Standard: 2, 10 ng/tube. Discard the ERBB2 stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours.
3. Human ERBB2 Biotinylated Antibody (100x): 1, 100 μl.
4. Avidin-Biotin-Peroxidase Complex (100x): 1 100 μl.
5. Sample Diluent: 1, 30 ml.
6. Antibody Diluent: 1, 12 ml.
7. Avidin-Biotin-Peroxidase Diluent: 1, 12 ml.
8. Color Developing Reagent (TMB): 1, 10 ml.
9. Stop Solution: 1, 10 ml.
10. Wash Buffer (25x): 1, 20 ml.
11. Plate Sealers: 4 Piece.

Storage

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature.)

Detection Range

62.5 pg/ml - 4000 pg/ml

Sensitivity

<10 pg/ml

General Description

CD's Human ERBB2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ERBB2 with a 96-well strip plate that is pre-coated with antibody specific for ERBB2. The detection antibody is a biotinylated antibody specific for ERBB2. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human ERBB2 protein as standards.
To measure Human ERBB2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450 nm is linearly proportional to Human ERBB2 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ERBB2 in the sample.