S. typhimurium Flagellin Protein

Specificity

Binds to human and mouse TLR5 (Toll-like receptor 5).

Bio-activity

TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.

Endotoxin

<0.01EU/μg purified protein (LAL test; Lonza).

Alternative Names

Flagellin; FliC

Purity

≥90% (SDS-PAGE)

Format

Lyophilized

Concentration

1mg/ml after reconstitution.

Buffer

Contains PBS.

Preservative

None

Storage

Short term at 2-8°C; long term storage at -20°C.
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Reconstitution

Reconstitute with 100μl sterile water.

Introduction

Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs) and can be detected by host cells through surface-localized toll-like receptor 6 (TLR6) and cytosolic Nod-like receptors (NLRs).

Keywords

Flagellin; FliC