E. Coli Verotoxin (Fecal) ELISA Kit

Sample

stool supernatant

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

This product is an in vitro procedure for the qualitative determination of verotoxin in feces. It is a double antibody (sandwich) ELISA using anti-verotoxin antibodies to capture the antigen from the stool supernatant.

Contents of Kit

1. Test Strips: Microwells rabbit anti-E. coli O157 antibodies-96 test wells in a test strip holder.
2. Reagent 1: One bottle containing 11 mL of monoclonal anti-verotoxin antibodies in a buffer with Thimerosal.
3. Reagent 2: One bottle containing 11 mL of an anti-mouse antibody conjugated to horseradish peroxidase in a buffer with Thimerosal.
4. Positive Control: One vial containing 2 mL of a verotoxin positive antigen in buffer.
5. Negative Control: One vial containing 2 mL of a buffer.
6. Chromogen: One bottle containing 11 mL of tetramethylbenzidine and peroxide.
7. Wash Concentrate: Two bottles containing 25 mL of concentrated buffer and surfactant with Thimerosal.
8. Stop Solution: One bottle containing 11 mL of 1 M phosphoric acid.

Storage

Reagents, strips and bottled components: Store at 2-8°C. For more detailed information, please download the following document on our website.

Sensitivity

Sensitivity=86%