Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Diamine Oxidase (DAO) activity in urine, serum, plasma, other biological fluids, tissue extracts, cell lysate, cell culture media samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 20 mL x 1 | 4°C |
| Enzyme | Powder x 1 | -20°C |
| Substrate | Powder x 1 | 4°C |
| Dye Reagent | Powder x 1 | 4°C |
| Dye Reagent Diluent | 1 mL x 1 | 4°C |
| Standard ( mmol/L) | 5 | 1 mL x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.05 mmol/L - 5 mmol/L
General Description
Diamine oxidase (DAO) is an enzyme that your body uses to break down ingested histamine. There are a wide variety of foods that contain histamine, and it is DAO's job to break this histamine down. DAO also helps with the integrity of the gut lining, protecting us from leaky gut and the functional digestive issues that can precipitate from it. The assay is initiated with the enzymatic catalysis of cadaverine by DAO. The enzyme catalyzed reaction products dianisidine can be measured at a colorimetric readout at 460 nm.
Citations
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