Creatinine Assay Kit

Sample

Urine, Serum, Plasma, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Creatinine content in urine, serum, plasma, other biological fluids samples.

Contents of Kit

Storage

2 - 8°C

Detection Range

0.05 mmol/L - 5 mmol/L

General Description

Creatinine is synthesized in the body at a constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function. Simple, direct and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in Research and Drug Discovery. Creatinine Microplate Assay Kit provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF. In the assay, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color, can be measured at a colorimetric readout at 546 nm.