APL 1beta-28 ELISA Kit

Sample

blood, plasma, tissues, CSF, (Extract samples)

Species Reactivity

N/A

Detection Method

cELISA

Intended Use

This kit is designed to measure the concentration of a specific peptide and its related peptides based on the principle of a "competitive" enzyme immunoassay.

Contents of Kit

1. EIA assay buffer concentrate (20×, 50ml)
2. Pre-coated EIA plate (96 wells, 1 plate)
3. Acetate plate sealer (APS) (3 pieces)
4. Primary antibody (1 vial)
5. Standard peptide (1 vial)
6. Biotinylated peptide (1 vial)
7. Positive control (2 vials)
8. Streptavidin-horseradish peroxidase (SA-HRP) concentrate (30μl)
9. Substrate solution (12ml)
10. 2N HCl (Stop solution) (15ml)

Storage

1. Store the kit at 4°C upon receipt. Do not freeze.
2. It is highly recommended that all solutions be used as soon as possible after reconstitution.
3. Any unused strips/columns may be removed from the pre-coated immunoplate. Please place strips back in the original zip-lock foil pouch with a dessicant, reseal, and store at 4°C. Do not allow moisture to accumulate on the wells.
4. If necessary, store the 1x assay buffer at 4°C.
5. If necessary, store any reconstituted solutions of standard peptide, biotinylated peptide, antibody and SA-HRP at 4°C.