Anti-Trenbolone polyclonal antibody

The subject of this study was photodegradation of boldenone and trenbolone belonging to the class of compounds which demonstrate endocrine disrupting activity. Their decomposition was carried out on the pathway of 254 nm photolysis alone and in the presence of hydrogen peroxide. The influence of pH, oxygen content in the reaction mixture, initial substrate concentration, photon fluence rate and initial concentration of H2O2 on the reaction rate was investigated. The effect of radical scavenger on the reaction course was also tested. The obtained results were used to determine kinetic parameters. Quantum yields of boldenone and trenbolone decay were equal to 0.61 +/- 0.02 and 0.0029 +/- 0.0002, respectively. The estimated rate constant of the reaction of hydroxyl radicals with trenbolone was (4.3 +/- 0.8) x 10(9) M-1 s(-1). (C) 2010 Elsevier B.V. All rights reserved.

In veal calf production androgens, estrogens and glucocorticoids are used to stimulate growth. However, sexhormones and glucocorticoids also influence the function of the immune system. From studies in humans and mice, androgens are known as immunosuppressive, while estrogens stimulate the production of antibodies and glucocorticoids also enhance the T-helper 2 response. To investigate whether the adaptive immune system is influenced by hormone administration, calves were treated with a hormone cocktail containing androgens, estrogens and glucocorticoids and vaccinated against Mycobacterium avium spp. paratuberculosis. The activity of the adaptive immune system was measured by using an antigen specific elispot assay (ES), lymphocyte stimulation test (LST) and an enzyme-linked immuno sorbent assay (ELISA). The results showed that the hormone treatment did not lead to significant differences in the function of the adaptive immune system between the hormone treated and the not hormone treated group while growth was stimulated in the hormone treated group. (c) 2007 Elsevier Ltd. All rights reserved.