Animals were immunized with C3H/HeJ thymocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with mature (medullary) thymocytes, pluripotential stem cells, platelets or cells of monocyte-macrophage lineage. IgG antibody producing cells are strongly positive for the J11d.2 antigen. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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