Animals were immunized with SVT-40, an SV40 transformed BALB/c cell line. Spleen cells were fused with NS-1 mouse myeloma cells. The antibody recognizes a denaturation sensitive determinant unique to large T (tumor) antigen. The antibody reacts with lower affinity than PAb 101 and precipitates large T antigen and NVT antigen (when complexed) but not small t antigen or the SV-80 small t antigen. The antibody does not bind to denatured large T or mouse NVT in radioimmunoassays. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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