Anti-Salmonella Polyclonal antibody

In the modern lifestyle, people are prone to disease. The epidemiology study shows that the lung and colon cancer leading position in both the gender around the world at any group. As based on the traditional systems of medicine, the plants can be used for the discovery of new medicine. The aqueous and ethanol extract of Nigella sativa (N. sativa) seeds were evaluated for their genotoxicity using Ames test and cytotoxicity by MTT assay. Ames assay was conducted using strains of Salmonella typhimurium TA-100, whereas MTT assay was performed on human alveolar basal epithelial cells (A549) and human colon cancer cell line (HCT15). There is no mutagenic activity was obtained when treated with aqueous and ethanol extract of Nigella sativa seeds. But both the extracts were found to be cytotoxic against the tested cancer cell lines. On the basis of the results, it was concluded that aqueous and ethanol extracts of Nigella sativa seeds possess cytotoxic potential but not mutagenic. It is suggested to explore the seeds further to evaluate its anticancer activity in the in-vivo model.

In this study, a series of unsymmetrical N,N-disubstituted benzimidazolium salts were synthesized as N-heterocyclic carbene (NHC) (2a-2j) and their Ag-I complexes. Their structures were characterized by elemental analyses, H-1 and C-13 nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy techniques. Further, both the salts and their silver(I)-NHC complexes have been evaluated for their potential antibacterial properties against a panel of bacterial strains namely, Micrococcus luteus, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus and Pseudomonas aeruginosa. The results show that silver complexes are effective against Salmonella Typhimurium, Listeria monocytogenes, and Micrococcus luteus with moderate to high activity, and their minimum inhibitory concentrations ranging from 0.0034 to 1.26 mg mL(-1). In addition, the benzimidazoles salts 2a-2j and silver-NHC complexes 3a-3j were screened for their antitumor activity. Complex 3e exhibited the highest antitumor effect with half-maximal inhibitory concentration (IC50) value of 4.2 mu g mL(-1) against MCF-7.