Established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an igg2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells confirmed as mouse with IEF of MDH, NP. . Viruses: ELISA: reverse transcriptase negative
Morphology
single cells in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1: 5, every 2-3 days; seed out at 0.5-5 x 10^6 cells/ml; Harvest: maximal density of about 1-2 x 10^6 cells/ml.
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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