Established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an igg1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen confirmed as mouse with IEF of AST, NP, PEP B. Cytogenetics: predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells 140(134-155). Viruses: ELISA: reverse transcriptase positive
Morphology
single cells growing in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1: 2, every 2-3 days; Maximal density at 1.0 x 10^6 cells/ml ; Harvest: 0.5-1.0 x 10^6 cells/ml.
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