Endothelial cells from mouse mesenteric and peripheral lymph nodes
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Myeloma
Sp2/0-Ag14
Clone
NFDB-90
Cell Line Description
Animals were immunized with endothelial cells from mouse mesenteric and peripheral lymph nodes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer). Unlike MECA-367, MECA-89 does not inhibit alpha 4, beta 7 dependent binding in vitro.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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