Animals were immunized with the Peyer's patch HEV binding lymphoma line TK1. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody recognizes the alpha subunit of a 160000/130000 dalton cell surface heterodimer expressed on lymphocytes and lymphomas capable of binding to high endothelial venules (HEV) of murine Peyer's patch. This subunit corresponds to the alpha subunit of the human VLA-4 integrin receptor, and the two are structurally closely related (if not identical).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
My Review for Rat Anti-LPAM-1 (Lymphocyte Peyer's Patch HEV Adhesion Molecule), VLA-4 Heterohybridoma [S2-3]
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