Purified recombinant human IL-1 beta (170500 dalton form)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8.653
Clone
JMC2-I45
Cell Line Description
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor. The affinity constant of it was measured to be 3.3 X 10^10 liter/mol.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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