Animals were immunized with purified human myeloma immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody against human IgG4 (Fc).Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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