Anti-Histidine polyclonal antibody

Michelia champaca known as champaca is belonging to family of Magnoliaceae. The present work consist of extraction and evaluation of anti-asthmatic activity of flowers of Michelia champaca Linn. Flowers was evaluated for anti-histaminic activity using isolated goat tracheal chain preparation and histamine induced Bronchoconstriction in Albino mice. Michelia champaca Linn. expressively inhibited dose reliant contraction of goat tracheal chain produced by histamine and also exhibited significant protection by prolonging preconvulsion dyspnea time (PCD) in mice. Thus, Michelia champaca Linn. showed anti-allergic activity against histamine and hence possesses potential role in the treatment of asthma.

An improved understanding of fundamental physiological principles and progressive pathophysiological processes in human articular joints (e.g., shoulders, knees, elbows) requires detailed investigations of two principal cell types: synovial fibroblasts and chondrocytes. Our studies, done in the past 8-10 years, have used electrophysiological, Ca(2+)imaging, single molecule monitoring, immunocytochemical, and molecular methods to investigate regulation of the resting membrane potential (E-R) and intracellular Ca(2+)levels in human chondrocytes maintained in 2-D culture. Insights from these published papers are as follows: (1) Chondrocyte preparations express a number of different ion channels that can regulate their E-R. (2) Understanding the basis for E(R)requires knowledge of (a) the presence or absence of ligand (ATP/histamine) stimulation and (b) the extraordinary ionic composition and ionic strength of synovial fluid. (3) In our chondrocyte preparations, at least two types of Ca2+-activated K(+)channels are expressed and can significantly hyperpolarize E-R. (4) Accounting for changes in E(R)can provide insights into the functional roles of the ligand-dependent Ca(2+)influx through store-operated Ca(2+)channels. Some of the findings are illustrated in this review. Our summary diagram suggests that, in chondrocytes, the K(+)and Ca(2+)channels are linked in a positive feedback loop that can augment Ca(2+)influx and therefore regulate lubricant and cytokine secretion and gene transcription.