Animals were immunized with a transfected NIH 3T3 cell line, 18-3-7 that expresses full length normal human neu protein. Spleen cells were fused with SP2/O myeloma cells . The antibody recognizes substantially purified p100, human neu related protein, the full-length HER-2/neu molecule in tumor tissue (p185) or the extracellular domain (ECD) (p105) in serum, plasma, cell cultures and fluids. The TA-1 (HB-10206) and NB-3 (HB-10205) antibodies recognize two distinct epitopes on the human neu gene product [U.S. Pat. 5, 401, 638].
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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