Anti-C. perfringens Polyclonal antibody

The effect of dietary lignocellulose on broilers performance, intestinal microbiota and morphology, pH of digesta and litter humidity after 28 and 42 days of the experiment was evaluated. A total of 384 Cobb500 chickens (initial weight: 41.88 +/- 1.56 g) were divided into 4 groups with 24 replications and fed with control diet (C), a control diet with added 0.4% of lignocellulose (T1), a diet with added 0.6% of lignocellulose at the expense of soybean meal and maize (T2), and a diet supplemented with 0.6% of lignocellulose at the expense of soybean meal (T3). T2 treatment significantly influenced body weight, weight gain (WG), feed intake (FI) and feed conversion ratio (FCR). T2 and T3 treatment increased average LAB and Bifidobacterium spp. count, and decreased the number of Escherichia coil in the ileum and cecum, while differences in cecal Clostridium perfringens count among 0.4% and 0.6% treatments were not observed. Feeding the lignocellulose diet did not affect the relative weights of empty proventriculus, gizzard or intestines, but led to a decrease in pHs. T3 treatment caused an increase of the villi heights and significantly lower moisture content in the litter. Even though the addition of lignocellulose into broilers diet positively influenced performances, changes in intestinal microbiota and villi heights, based on the results of the present study, supplementation with 0.6% lignocellulose is recommended.

The current study was carried out in all administrative divisions of the province to explore the prevalence of Clostridium perfringens type D in small ruminants. A total of 204 fecal samples were collected from sheep and goats suffering from seasonal diarrhea. C. perfringens type D was identified through different biochemical tests and PCR. Antibiograms and lab animal trials were also carried out on local isolates of C. perfringens type D. Out of 204 analyzed samples, 89.18 % were found positive while 10.82 % were negative for C. perfringens type D. The division wise distribution showed that Quetta, Zhob, Sibi and Kalat were more effected zones of the Balochistan province. Sheep were more effected (54.78 %) than goats (34.4 %). C. perfringens was identified through different biochemical tests and PCR. Based on length of synthesized fragments by PCR, toxin gens of alpha and epsilon types were detected. C. perfringens type D was sensitive to tetracycline, ciprofloxacin, vancomycin, kanamycin, streptomycin, gentamicin, sulfamethoxazole and erythromycin, while showed resistance against carbenicillin, amoxicillin, penicillin g, colistin sulphate, bacitracin and metronidazole. In lab animal trial C. perfringens type D affected the fore stomach, intestine, liver, kidney, pancreas and gall bladder of mice.