Metastatic human epidermoid carcinoma (HEp-3) cells by subtractive immunization
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
NS0
Clone
52-3
Cell Line Description
Animals were immunized with metastatic human epidermoid carcinoma (HEp-3) cells by subtractive immunization. Spleen cells were fused with NS0 myeloma cells.The antibody identifies SIMA135/CDCP1 (subtractive immunization M(+)HEp3 associated 135 kDa protein/CUB domain containing protein 1)
Application Notes
Treating and diagnosing metastatic tumors.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Synonyms
9030022E12Rik;AA409659;CD 318;CD318;CD318 antigen;CDCP 1;Cdcp1;CDCP1_HUMAN;CUB Domain Containing Protein 1;CUB domain-containing protein 1;E030027H19Rik;FLJ13772;FLJ22375;FLJ22969;Membrane glycoprotein gp140;MGC31813;OTTHUMP00000164120;OTTHUMP00000209568;RGD1305578;SIMA 135;SIMA135;Subtractive immunization M plus HEp3 associated 135 kDa protein;Subtractive immunization M plus HEp3-associated 135 kDa protein;Transmembrane and associated with src kinases;TRASK;
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