Amoxicillin [KLH]

Name: Amoxicillin [KLH]
SKU: DAG4477
Rating: 5 (1 reviews)

Amoxicillin, KLH-conjugated, synthetic

COA

SDS

Datasheet

Summary

Product Overview

Amoxicillin, KLH-conjugate

Target

Amoxicillin

Nature

Synthetic

Tag/Conjugate

KLH

Format

Liquid

Concentration

Approximately 2.0 mg/mL KLH

Buffer

Supplied in 20 mM PBS, pH 7.4

Preservative

None

Storage

Keep below -20°C for up to 1 year. Avoid repeated freeze-and-thaw. For short term storage (< 3 weeks) keep at 4°C.

Introduction

Amoxicillin is a?penicillin?in which the substituent at position 6 of the?penam?ring is a 2-amino-2-(4-hydroxyphenyl)acetamido group. It has a role as an antibacterial drug. Amoxicillin Anhydrous is the anhydrous form of a broad-spectrum, semisynthetic aminopenicillin antibiotic with bactericidal activity. Amoxicillin binds to and inactivates?penicillin-binding proteins (PBPs) located on the inner membrane of the bacterial cell wall. Inactivation of PBPs interferes with the cross-linkage of?peptidoglycan?chains necessary for bacterial cell wall strength and rigidity. This interrupts bacterial cell wall synthesis and results in the weakening of the bacterial cell wall and causes cell lysis.

Antigen Description

The amoxicillin and KLH (keyhole limpet hemocyanin) (10 mg each) are conjugated by EDC method in 0.1 M MES pH 5.0. The carboxyl group in the amoxicillin is directly linked to an amine group in the KLH, and/or a carboxyl group in the KLH is directly linked to the amine group in the amoxicillin, without any linker by EDC conjugation method. Given the molecular weights of amoxicillin and KLH are 365.4 Da and 8,000 – 9,000 kDa, respectively, the molar ratio of amoxicillin:KLH in the conjugation solution is 21894 - 24631:1. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS, pH 7.4. The number of amoxicillin that is actually conjugated to each KLH molecule is not determined. Note: Due to its high molecular weight and its tendency to form aggregates, the conjugate is not completely soluble in the buffer that it is in. Therefore, it is strongly recommended to vigorously vortex immediately prior to aliquot or use.

Keywords

Amoxicillin; amox; AMOXICILLIN T TRIHYDRATE; AMOXICILIN TRIHYDRATE; AMOXYCILLIN TRIHYDRATE; abicyclo[3.2.0]heptane-2-carboxylicacidtrihydrate; alpha-amino-p hydroxybenzylpenicillin; amolin; amopenixin; amoxi; amoxicillinstandardsolution; amoxicillintrhydr

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References

Severe cutaneous adverse drug reactions: Incidence, clinical patterns, causative drugs and modalities of treatment in Assiut University Hospital, Upper Egypt

DERMATOLOGIC THERAPY

Authors: Abou-Taleb, Doaa A. E.; El-Sayed, Ahmed M.; Ghabesha, Ayad A.; Hassan, Sahar B.

Abstract

Stevens-Johnson syndrome (SJS), toxic epidermal necrolysis (TEN), and overlap SJS/TEN are life-threatening diseases that are most frequently caused by drugs. Much debate remains about the role of systemic corticosteroids (SCs) in their treatment. Our aim to determine the incidence, causative drugs, the role and side effects of SCs in severe cutaneous adverse reactions (SCARs), in Assiut University Hospital (AUH). Patients This study was conducted in Department of Dermatology at AUH, from 2012 to 2017. All patients with SJS, overlap SJS/TEN and TEN admitted during this period were included in the study. Eighty-three patients with SCARs were included in this study. The most common type was SJS (67.5%). The incidence ranged from 1.7% in 2012 to 7.7% in 2017. Carbamazepine, valproic acid, lamotrigine, diclofenac sodium, and flucloxacillin-amoxicillin were the most common causative drugs. The most common side effects of SCs were peptic ulcer (55.5%) and hypertension (51.8%). The mortality rate in patients treated with SCs was 100% in TEN, 33.3% in overlap SJS/TEN and 16.3% in SJS. The patients of SCARs must be aware of the causative drugs and must never be re-administered. SCs in treatment of SCARs may increase the complications and the mortality rate.

Ocular syphilis with optic disc neovascularization treated with bevacizumab evaluated by OCT angiography and electroretinography

JOURNAL OF OPHTHALMIC INFLAMMATION AND INFECTION

Authors: Miura, Gen; Baba, Takayuki; Takeishi, Marie; Tatsumi, Tomoaki; Yokouchi, Hirotaka; Yamamoto, Shuichi

Abstract

We present our findings in an atypical case of ocular syphilis with optic disc neovascularization that was treated with intravitreal bevacizumab and followed by multimodal imaging and electroretinography. A 29-year-old man presented with a chief complaint of night blindness of one-year duration. Our initial examination showed that an optic disc neovascularization was present, and the optical coherence tomographic (OCT) images showed a reduction in the length of the ellipsoid zone of both eyes. Fluorescein angiography showed leakage from the optic disc neovascular tissue, and the presence of nonperfused areas in the peripheral retina. Blood test was strongly positive for syphilis. He was administered oral amoxicillin and prednisolone. He was also treated with an intravitreal injection of bevacizumab which led to a rapid suppression of the neovascularization. However, panretinal photocoagulation had to be performed because OCT angiography and fluorescein angiography detected residual neovascularization. Although these treatments suppressed the activity of the ocular syphilis, electrophysiological improvements were not seen even 1 year after the initial treatment. OCT angiography and electroretinogram are useful techniques for monitoring the effectiveness of the treatments.