Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Alanine Transaminase (ALT) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Substrate | Powder x 1 | 4°C |
| Dye Reagent I | 5 mL x 1 | 4°C |
| Dye Reagent II | 10 mL x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Standard | Powder x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.625 umol/mL - 20 umol/mL
General Description
Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (GPT), facilitates the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Alanine Transaminase Activity Microplate Assay Kit provides a simple and direct procedure for measuring alanine transaminase activity in a variety of samples. This kit is based on the hydrolysis of substrate, reaction product phenylhydrazone can be measured at a colorimetric readout at 520 nm, which is directly proportional to the amount of alanine transaminase activity.
Citations
Publication ()
Have you cited DFLA26002 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
