Description
Polyinosinic:polycytidylic acid (poly I:C) is an immunostimulant. It is used in the form of its sodium salt to simulate viral infections. Poly I:C is known to interact with toll-like receptor (TLR) 3, which is expressed in the membrane of B-cells, macrophages and dendritic cells. Poly I:C is structurally similar to double-stranded RNA, which is present in some viruses and is a "natural" stimulant of TLR3. Thus, Poly I:C can be considered a synthetic analog of doublestranded RNA and is a common tool for scientific research on the immune system Double-stranded RNA, polyriboinosinicpolyribocytidylic acid (poly IC), acts as an adjuvant that enhances adaptive immune responses.
Poly IC induces the secretion of type I IFNs, proinflammatory cytokines, and the maturation of DC. TLR3 recognizes poly IC in the endosome and initiates signaling through Toll/IL-1R domaincontaining adaptor inducing IFN-β (TRIF). There is a TLR3- independent cytoplasmic pathway for poly IC recognition. Retinoic acid-inducible gene I (RIG-I) and melanoma differentiationassociated gene 5 (Mda5) represent a family of RNA helicases that sense viral RNA in the cytoplasm. RIG-I recognizes the 5'- triphosphate end of RNA in various RNA viruses, whereas Mda5 recognizes poly IC. RIG-I and Mda5 signaling solely depends on an adaptor, IFN-β promoter stimulator 1 (IPS-1) (MAVS/Cardif/VISA). Together, the host innate immune system employs at least two pathways for poly IC recognition
Solubility: Soluble in water up to 10 mg/ml.
Working concentration: 10-100 mg/mouse
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