Plasmodium Falciparum HRP2 ELISA Kit

Sample

Whole blood, culture supernatant, serum or plasma samples and other preparations containing HRP2

Detection Method

sELISA

Intended Use

The PfHRP2 Malaria is a quantitative ELISA based on the original Malaria Ag CELISA assay. Using the same anti-P.falciparum (P.f) monoclonal antibodies as the Malaria Ag CELISA. This assay was developed to provide improved sensitivity for quantifying HRP-2 to the picogram-level with a lower detection limit at approximately 0.01 ng/mL. Samples of whole blood, culture supernatant, serum or plasma samples and other preparations containing HRP2 can be used. This test can also be used as a confirmatory test for falciparum malaria in situations where the traditional diagnosis is unclear, for screening blood transfusion products or to confirm cases of travel-related infection. It is not intended to replace the conventional blood film diagnosis.

Contents of Kit

QUMW CELISA Plate – 1x96 wells - (single use strips) 2 plates
Conrol + Standard (freeze-dried) 1 x 0.5 mL
Control - Negative Control 1 x 5.0 mL
QUPO Enzyme Conjugate (200 x) 1 x 0.12 mL
QUCD Conjugate Diluent 1 x 24 mL
QUPT PBS/Tween (20x) 1 x 125 mL
QUSC Substrate Chromogen (TMB) (20x) 1 x 1.2 mL
QUSB Substrate Buffer 1 x 24 mL
QUSS Stopping Solution 1 x 12 mL

Storage

Store all components at 2-8°C

Sensitivity

0.01 ng/mL