Pig NSE (Neuron-Specific Enolase) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Pig

Detection Method

sELISA

Intended Use

In vitro quantitative determination of NSE concentrations in serum, plasma, cell culture supernatant and other biological

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	96T(8×12)	2-8°C/-20°C
2	Lyophilized Standard	2vial	2-8°C/-20°C
3	Biotin-labeled Antibody (Concentrated, 100×)	120 ul	2-8°C
4	HRP-Streptavidin Conjugate (SABC, 100×)	120 ul	2-8°C
5	TMB Substrate	10 ml	2-8°C
6	Sample Dilution Buffer	20 ml	2-8°C
7	Antibody Dilution Buffer	10 ml	2-8°C
8	SABC Dilution Buffer	10 ml	2-8°C
9	Stop Solution	10 ml	2-8°C
10	Wash Buffer (25×)	30 ml	2-8°C
11	Plate Sealer	5	-

Storage

Store at 2-8°C (for sealed box), please do not freeze! See kit label for expiry date.
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.