Phosphorylated Neurofilament NF-H ELISA Kit

Sample

Plasma, serum, CSF and tissue extracts.

Species Reactivity

Human; Mouse; Rat; Chicken; Cow; Horse; Pig

Detection Method

sELISA

Intended Use

Phosphorylated Neurofilament NF-H ELISA Kit is an Enzyme Immunoassay kit for the quantification of Phosphorylated Neurofilament NF-H in plasma, serum, CSF and tissue extracts. For laboratory research only, not for drug, diagnostic or other use.

Contents of Kit

Store unopened kit at 2-8°C. HRP-Anti-pNF-H Antibody (100×)should be stored at -20°C upon receipt. Use the kit before expiration date.

Storage

Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.

Precision

The CV value of intra-assay precision was <6% and inter-assay precision was <10%.

Detection Range

The standard range can cover form 0.156 – 10 ng/ml.

Sensitivity

70 pg/ml

General Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. The pNF-H protein has been detected in large amounts following experimental spinal cord and brain injury in rats. Levels of greater than 100ng/mL of pNF-H were detectable in blood samples following serious spinal cord injury and lower but still easily detectable levels were seen in blood of animals given experimental brain injury.
More recent studies have revealed considerable amounts of this protein in the blood of transgenic mice carrying mutations of human copper/zinc superoxide dismutase-1 which are associated with amyotrophic lateral sclerosis. These mice develop an axonal degeneration pathology similar to that seen in humans with ALS, and blood pNF-H levels can be used to monitor progression of the disease. Interestingly, pNF-H was detectable before the onset of obvious disease symptoms.

Standard Curve