Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. In the U.S., the National Primary Drinking Water Regulations (NPDWRs) limit for the action level of lead ion contaminants in drinking water is 15 ng/mL. Long-term health consequences of drinking lead-contaminated water include kidney problems and high blood pressure for adults, and the physical and mental development delays in infants and children. Lead contamination in water has attracted significant attention around the World, and fast and sensitive methods for monitoring water quality are for protecting human and animal health in great demand. The lateral flow assay (LFA) has emerged as a powerful analysis platform for detecting various analytes including small molecules, such as pesticides, biotoxins and heavy metals, due to its speed, simplicity and low-cost characteristics.