Parvovirus IgG ELISA Kit

Name: Parvovirus IgG ELISA Kit
SKU: DEIA-NS2401-26
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Parvovirus B19 IgG ELISA is a quantitative and qualitative test for detection of human antibodies in serum or plasma against Parvovirus B19. For sale in the U.S. for Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96), 1 frame, the coating material is inactivated. 12
2. Standard serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus -surface antigen) and anti-HCV-Ab; preservative: < 0.1% sodium azide, colouring: Amaranth O. 2 × 2 ml
3. Negative control serum (ready-to-use), Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus -surface antigen) and anti-HCV-Ab; preservative: < 0.1% sodium azide, colouring: Lissamine green V. 2 ml
4. Anti-human-IgG-conjugate (ready-to-use), Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane.13 ml
5. Washing solution concentrate (sufficient for 1 litre), Sodium chloride solution with Tween 20, 30 mM Tris preservative: < 0.1% sodium azide, 33.3 ml
6. Dilution buffer or specific IgM dilution buffer Parvovirus B19, Phosphate buffer with protein and Tween 20; preservative: < 0.1% sodium azide, 0.01 g/l Bromphenol blue sodium salt, E. coli-lysate / specific IgM dilution buffer. 2 × 50 ml
7. Stopping solution, 1.2 N sodium hydroxide. 15 ml
8. Substrate (ready-to-use), Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow color. This does not reduce the quality of the product!) 13 ml.

Storage

Reagent	Storage	Stability
microtiter strips (antigen)	after opening at 2-8°C in closed aluminum-bag with desiccant strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions!	4 weeks
control sera / standard sera	after opening at 2-8°C	until expiry date; 24 months from date of production
conjugate	ready-to-use solution, at 2-8°C Avoid contamination (sterile tips!)	until expiry date; 28 months from date of production
dilution buffer	after opening at 2-8°C. Discard cloudy solutions! unopened	24 months until expiry date; 36 months from date of production
Specific IgM dilution buffer Parvovirus B19	after opening at 2-8°C. Discard cloudy solutions! unopened	24 months until expiry date; 36 months from date of production
washing solution	concentrate after opening at 2-8°C working dilution at 2-8°C working dilution at room temperature bottles used for the working dilution should be cleaned regularly, discard cloudy solutions	until expiry date 2 weeks 1 week
substrate	ready-to-use solution at 2-8°C, protected from light! Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25).	until expiry date; 24 months from date of production
stopping solution	after opening at room temperature	until expiry date

General Description

Parvovirus B19 is the most important pathogen for clinically relevant human infections with Parvoviruses. The virus replicates in dividing human erythroid cells.
Parvovirus B19 is ubiquitous. In industrialized countries 2-10% of children younger than 5 years had an infection and 40-60 % of individuals older than 20 years show Parvovirus B19 antibodies. Parvovirus B19 infections are mainly transmitted by naso-pharyngal secretion (droplet infection), due to close contact with infected individuals. In 5% of pregnant women the infection is transmitted to the fetus, leading to different types of fetopathy. In the first and second trimesters the risk of fetal abortion is highest. Transmission of Parvovirus B19 infections by blood or plasma donation in the phase of viremia was rarely reported. ELISA test-systems are especially suited for the detection of Parvovirus B19 IgM and IgG due to their accurate immunoglobulin class specific analysis of the immune response.

Citations