The CD p53 autoantibodies ELISA shows a high signal to noise ratio. Rohayem et al. evaluated the potential of three commercial p53 autoantibodies ELISAs to provide correct diagnostic classification by use of ROC curve analysis using sera of 72 patients with suspicion of malignancy and sera of 72 healthy blood donors as control. Among the three tests used, the solid-phase ELISA (CD) showed the highest diagnostic accuracy (AUC = 0.902) with a significant difference from other tests. CD offers an assay, which enables easy quantitative determination of p53 autoantibodies. Therefore you are able to monitor patient specific levels of p53 antibodies during the course of the disease or during therapeutic manipulations.
Storage
All unopened reagents are stable until expiration date if stored at 2-8°C .
General Description
The p53 gene is altered in up to 60% of most types of human cancer arising from a wide spectrum of tissues. This leads to abnormal p53 protein accumulation inside the cancer cell. Some patients with an abnormal p53 function develop in a yet unclear mechanism an immunresponse against p53 protein. p53 autoantibodies gain increasing attention since several studies described their detection in sera from patients with various malignancies (e.g., colorectal, ovarian, oral, head & neck, trophoplastic and lung carcinomas). The presence of p53 autoantibodies seems to be associated with more progressive cancers and reduced disease-free survival of (surgically) treated patients. There is accumulating evidence that p53 autoantibodies may be indicative for a poor prognosis and a higher risk of tumor relapse. p53 autoantibodies have a useful potential in patient monitoring during therapeutical follow-up. A postoperative significant drop in p53 autoantibodies may be the result of complete tumor resection and successful (adjuvant) chemotherapy.
Standard Curve
Dilute the calibrator (10U/ml) with sample dilution buffer. Use undiluted, diluted calibrator and zero control for calculating the calibration curve. We recommend 4-6 measurements and an additional zero control. The absorption (E450) of undiluted calibrator corresponds to exact 1 Unit (1U/test).
Citations
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COA
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