Ovalbumin (OVA) Epitope Peptide Antibodies

Ovalbumin (OVA), a major allergen protein present in egg whites, is also widely used as a model antigen in immunological studies due to its immunostimulant properties. Many researchers have reported T-cell and B-cell epitopes of OVA in the context of allergic immune responses. Due to its particularly valuable for studying T cell responses, OVA epitope peptides have also been extensively utilized in cancer immunotherapy and vaccine development.

Fig. 1 Epitope mapping on OVA protein.
(Suprun M et al. Int Arch Allergy Immunol. 2022)

OVA Peptides in Allergy

OVA is widely used as a model allergen for studying IgE-mediated hypersensitivity and food allergies. A number of studies have reported the partial T and B cell epitope mapping of OVA using murine models and allergic patients' sera. Analysis of IgE binding epitopes in mice provides potential strategies for design of specific immunotherapy.

OVA323-339 (ISQAVHAAHAEINEAGR)

This MHC class II epitope is recognized by CD4+ T helper cells and is crucial for orchestrating adaptive immune responses. As an immunodominant epitope of OVA, OVA323-339 accounts for 50% of the OVA-specific B-cell response and 60–70% of the OVA-specific T-cell response. It is reported that OVA323-339 can induce Th2-dominant immune responses, and the splenocyte proliferative response to OVA323-339 is possibly IgE-related much like that of OVA. Research on this peptide enhances understanding of how T helper cells contribute to allergic inflammation and antibody production.

Other Important OVA Peptides

Researchers have mapped and analyzed human patients and BALB/c mouse IgE epitopes present on the ovalbumin protein. These important epitopes are summarized in Table 1. In addition, In the BALB/C mouse model, the structural heterogeneity of IgE epitopes generated after exposure to an allergen through different routes (oral, intraperitoneal and subcutaneous entry route) also have been characterized (Table 2). Such research can be exploited in the development of peptide-based vaccines, the production of recombinant hypoallergenic molecules or the determination of cross-reactivity between food allergens.

Product Performance

Creative Diagnostics offers a variety of antibodies against OVA epitope peptides (aa 61-68, aa 191-202, aa 257-264, aa 323-339, aa 363-374, etc.) to assist your scientific research. These antibody target widely reported epitopes or epitopes similar to IgE epitopes in human patients. Our products cover a wide range of applications and can be conjugated with various labels according to customer needs. Product performance is as follows.

Fig.3 Degranulation of mast cells by monoclonal IgE antibody. Comparing the three antibodies (Left); Dose response effect of A8102 and A8103 (Right).
(Cat. No CABT-L0502Y, CABT-L0504Y, CABT-L0503Y)