ODN M362 Adjuvants

1 mg of lyophilized ODN M362 Vaccine grade adjuvant

10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

ODN M362 is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.

- Upon resuspension, prepare aliquots of ODN M362 and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

5'-tcgtcgtcgttc:gaacgacgttgat-3' (25 mer)

Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.

Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses.

Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN M362 is a type C CpG ODN specific for human/mouse TLR9. Type C CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. Type C CpG ODNs are potent inducers of IFN-α from pDC and strong B cell activators. In vivo studies have demonstrated that type C ODNs which combine the effects of types A and B ODNs, such ODN M362, are very potent Th1 adjuvants.

Add 500 μl endotoxin-free physiological water to the 1 mg ODN M362 vial to obtain a solution at 2 mg/ml.

- Mix the solution by pipetting up and down.