ODN 1668 Adjuvants

Product Name

Cat.No.

Content

Storage and Stability

ODN 1668 is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year when properly stored.

- Upon resuspension, prepare aliquots of ODN 1668 and store at -20°C. Resuspended product is stable 6 months when properly stored. Avoid repeated freeze-thaw cycles.

ODN 1668 Sequence

5'-tccatgacgttcctgatgct-3' (20 mer)

Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.

Description

Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs), such as ODN 1668, have been extensively studied as adjuvants. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by TLR9, which is expressed exclusively on human B cells and plasmacytoid dendritic cells (pDCs), thereby inducing Th1-dominated immune responses. Pre-clinical studies, conducted in rodents and non-human primates, and human clinical trials have demonstrated that CpG ODNs can significantly improve vaccine-specific antibody responses.

Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities. Furthermore, CpG ODNs activate TLR9 in a species-specific manner. ODN 1668 is a type B CpG ODN specific for mouse TLR9. Type B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides and can strongly activate B cells.

ODN 1668 has been tested as an adjuvant in numerous animal models. Research in mice demonstrated that ODN 1668 administration can induce the activation of antigen presenting cells and type I IFN anti-viral activity, indicative of a Th1 immune response.

Preparation of Sterile Stock Solution (2 mg/ml)

Add 500 μl endotoxin-free physiological water to the 1 mg ODN 1668 vial to obtain a solution at 2 mg/ml.

- Mix the solution by pipetting up and down.

Working Concentration