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Recombinant Antigens for Mycobacterium Tuberculosis Diagnosis

Tuberculosis (TB) is a contagious and often severe airborne disease caused by infection with Mycobacterium tuberculosis bacteria. TB usually affects the lungs, but can also affect other parts of the body. Without treatment, TB can spread to other parts of the body through the bloodstream. A total of 1.5 million people died from TB in 2020 (including 214 000 people with HIV). Worldwide, TB is the 13th leading cause of death and the second leading infectious killer after COVID-19. With years of protein and antigen production experience and advanced facilities, Creative Diagnostics offers a large range of Mycobacterium tuberculosis antigens which have been validated to work in various types of immunoassays.

Performance

  • Detects the M. Tuberculosis antibody. Suitable for EIA, CLIA, LF
  • >90% pure (SDS-PAGE), manufactured in large scale
  • Minimal batch-to-batch variations

Product list

Cat. No Description Application
DAGA-168 Native M. Tuberculosis LAM Antigen EIA, CLIA, LF
DAGA-170 Recombinant M. Tuberculosis CFP-10 Antigen EIA, LF, Immunogen, Control
DAGA-180 Recombinant M. Tuberculosis MPT63 Antigen EIA, Immunogen
DAGA-187 Recombinant M. Tuberculosis ESAT-6 Antigen EIA, LF, Immunogen, Control
DAG-T2449 Recombinant M. Tuberculosis HSP (16 kDa) Antigen EIA, CLIA

Introduction

M. tuberculosis can be regarded as a conditional pathogenic bacterium in a sense because it only causes TB in immunocompromised hosts. Over 90% of M. tuberculosis-infected individuals can spontaneously control the infection. A key prerequisite for intracellular colonization of mycobacteria is their ability to adhere and enter host cells. Upon infection by M. tuberculosis, the host orchestrates multiple signaling cascades via the pattern-recognition receptors (PRRs) to launch a variety of innate immune defense functions, which are subverted by secreted M. tuberculosis effector proteins.

Schematic representation of the ESX-1 secretion system

Fig. 1 Schematic representation of the ESX-1 secretion system (Forrellad MA, et al. 2013)

  • ESAT6 (6-kDa early secretory antigenic target, also known as ESXA) and CFP10 (10-kDa culture filtrate protein, also known as ESXB) are two relevant proteins associated to virulence in MTBC that are secreted by T7SS. ESAT6 and CFP10 have been described as dominant antigens recognized by T-cells in natural infection in humans and bovines and in experimentally infected animals.

Schematic visualization of the structure of ManLAM

Fig. 2 Schematic visualization of the structure of ManLAM (Turner J, et al. 2018)

  • Lipoarabinomannan (LAM) is a lipoglycan and major virulence factor in the bacteria genus Mycobacterium. Its primary function is to inactivate macrophages and scavenge oxidative radicals. LAM is one of the most promising candidate antigens for detecting TB, and it has been used to develop commercially available immunoassays.

References

  1. Smith I. (2003). Mycobacterium tuberculosis Pathogenesis and Molecular Determinants of Virulence. Clinical Microbiology Reviews. 16(3), 463-496.
  2. Chai Q Y, Zhang Y, Liu C H. (2018). Mycobacterium tuberculosis: An Adaptable Pathogen Associated with Multiple Human Diseases. Frontiers in Cellular and Infection Microbiology. 8, 158.
  3. He X Y, Li J, Hao J, et al. (2011). Assessment of Five Antigens from Mycobacterium tuberculosis for Serodiagnosis of Tuberculosis. Clinical and Vaccine Immunology. 18: 565-570.
  4. Forrellad, M. A, Klepp, L. I, et al. (2013). Virulence factors of the Mycobacterium tuberculosis complex. Virulence, 4(1), 3–66.
  5. Turner J, Torrelles JB. (2018). Mannose-capped lipoarabinomannan in Mycobacterium tuberculosis pathogenesis. Pathogens and Disease. 76(4), fty026.
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