|Cat. No.||Product Name||Transgene||Description||Price|
|CSC-ACE01||Human HEK293T (ACE2) Stable Cell Line||Human ACE2||One frozen vial containing millions of cells.||Inquiry|
|CSC-ACE02||Human HEK293T (hACE2-hTMPRSS2) Stable Cell Line||
|One frozen vial containing millions of cells.||Inquiry|
Figure 1. Cell morphology of HEK293T-ACE2 cell line under bright field (left) and GFP fluorescence (right).
Figure 2. Infection assay. 72 h after infection of 20 μL SARS-CoV-2 S glycoprotein pseudotyped GFP lentivirus into HEK293T-ACE2 cells, under bright field (left) and GFP fluorescence (right).
Figure 3. qPCR analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.
Figure 4. Western blot analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.
Q: Does this cell line express full length protein on cell surface (805 amino acid is full length)?
A: Yes, it express full length human ACE2 protein.
Q: Did FACS analysis was done to ensure the protein is present on cell surface?
A: No. But our ACE2/HEK293T can be successfully infected by Pseudotyped GFP rSARS-CoV-2 Spike (CD Cat# COV-PS02) which means cells contain functional ACE2.
Q: Do CSC-ACE01 cell line require a selection antibiotic in the media? if so, which one?
A: We use 1 μg/mL of puromycin.
Q: How do you transduce ACE2 and TMPRSS2 into this cell line (CD Cat# CSC-ACE02)?
A: The cells were transduced using a second generation lentiviral vector carrying a transgene for hsACE2 (Accession# NM_021804.3) and hTMPRSS2 (Accession# NM_001135099.1), Integration and expression were selected for by incubation with Puromycin and Blasticidin.
Q: For CSC-ACE01, I was wondering how many times the ACE2 cell line could be passaged before the ACE2 receptors started to fall off?
A: Maintain the cells in Puromycin to ensure the ACE2 transgene is expressing. When grown under selection, the receptor levels should not decrease with passage. We have carried the ACE2 cell line to P30 without losing transduction efficiency.