Figure 1. Cell morphology of HEK293T-ACE2 cell line under bright field (left) and GFP fluorescence (right).
Figure 2. Infection assay. 72 h after infection of 20 μL SARS-CoV-2 S glycoprotein pseudotyped GFP lentivirus into HEK293T-ACE2 cells, under bright field (left) and GFP fluorescence (right).
Figure 3. qPCR analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.
Figure 4. Western blot analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.
|Cat. No.||Product Name||Description||Price|
|CSC-ACE01||Human ACE2 Stabel Cell Line - HEK293T||One frozen vial containing millions of cells.||Inquiry|
Q: Does this cell line express full length protein on cell surface (805 amino acid is full length)?
A: Yes, it express full length human ACE2 protein.
Q: Did FACS analysis was done to ensure the protein is present on cell surface?
A: No. But our ACE2/HEK293T can be successfully infected by Pseudotyped GFP rSARS-CoV-2 Spike (CD Cat# COV-PS02) which means cells contain functional ACE2.