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Human ACE2 Stable Cell Line - HEK293T

HEK293T/ACE2

CELL LINE INFORMATION

Cat.No.
CSC-ACE01
Cell Line Description
This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HEK293T cells, followed by stable cell selection. HEK293T is derived from HEK293 and is commonly used in scientific research. HEK293T-human ACE2 cell line can be used for in vitro screening and characterization of drug candidates against SARS-CoV, SARS-CoV-2 (2019-nCoV).
Background
ACE2 protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, this protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus).
Host Cell
HEK293T

SAFETY AND PACKAGING

Mycoplasma
Negative
Format
One frozen vial containing millions of cells
Storage
Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

  1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
  2. No eating, drinking or smoking while handling the stable line.
  3. Wash hands after handling the stable line and before leaving the lab.
  4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
  5. All waste should be considered hazardous.
  6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship
Dry ice

REPRESENTATIVE DATA

Figure 1. Cell morphology of HEK293T-ACE2 cell line under bright field (left) and GFP fluorescence (right).

Figure 2. Infection assay. 72 h after infection of 20 μL SARS-CoV-2 S glycoprotein pseudotyped GFP lentivirus into HEK293T-ACE2 cells, under bright field (left) and GFP fluorescence (right).

Figure 3. qPCR analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.

Figure 4. Western blot analysis of human ACE2 expression in HEK293T-ACE2, HEK293T is used as control.

Order Information

Cat. No. Product Name Description Price
CSC-ACE01 Human ACE2 Stabel Cell Line - HEK293T One frozen vial containing millions of cells. Inquiry

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