Creative Diagnostics offers the Kanamycin ELISA Kits for quantitative and qualitative analysis of kanamycin residue in animal tissues (muscle and liver, etc.) and milk etc. Each of our kits goes through fit-for-purpose validation and stability testing to ensure high accuracy, sensitivity and specificity.
Kanamycin ELISA Kits:
Kanamycin is an aminoglycoside antibiotic and is widely used in treating animal diseases. It harms the 8th cranial nerves, causing damages to the vestibular and cochlear. The main manifestations of renal toxicity are the damages of the proximal convoluted tubule, causing protein urine, hematuresis, renal hypofunction, etc. The residues of Kanamycin in animal derived food affect human health, in China and Occident, Kanamycin has been limited to use for its neurotoxicity and nephrotoxicity. Kanamycin residue in the production of biological products may lead to abnormal reactions of human beings, thus strict MRLs have been established. This kit is a rapid test product for the determination of tetracycline residues which is sensitive, accurate and time-saving. It can considerably reduce the operation errors in the assay.
The Kanamycin ELISA Kit is designed to detect Kanamycin based on the principle of "indirect-competitive" enzyme immunoassay. The microtiter wells are coated with capture BSA-linked antigen. Kanamycin in the sample competes with antigen coated on the microtitre plate for the antibody. After the addition of enzyme conjugate, chromogenic substrate is used and the signal is measured by spectrophotometer. The absorption is inversely proportional to the Kanamycin concentration in the sample. This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kanamycin antigen. Standards or samples are added to the appropriate microtiter plate wells with Kanamycin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Kanamycin and Kanamycin in standards or samples with the Kanamycin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Kanamycin in the standards or samples. The color development is stopped and the intensity of the color is measured.